I'm annoyed. Seems like my cell cultures got infested with bacteria. Again. It has to be the new media that I made up - I didn't realise I was meant to filter sterilise the serum before adding it to the media. Obvious, really, if you think about it. But well, I guess I didn't. What really got to me is that after the first bacterial assault on my poor defenseless cells, I incubated some of the media overnight with LB, and it was still clear the next day. I figured it was just me having done something stupid, e.g. sneeze on my cells, and it was safe to use the aforementioned (and henceforth damned) bottle of media - big mistake.. (oh, and on a random tangent, apparently it's possible to give your cells a yeast infection just by breathing on them after a night out drinking). Man.. as if I'm not already behind schedule with this project. Just hope I'll have something to show at the end of this.
So much for the best laid schemes of mice and men.
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